2X-121 is an investigational, orally-available small molecule targeted inhibitor of Poly ADP ribose polymerase (PARP), a key enzyme involved in DNA damage repair in cancer cells.
How it Works
The drug candidate has a novel dual-inhibitory action against both PARP 1/2 and Tankyrase 1/2 (important regulators of canonical Wnt/β-catenin, a critical checkpoint in metastases, particularly in triple-negative breast cancer).
The molecule is also active in P-glycoprotein expressing cells, suggesting it may overcome PARP inhibitor resistance.
Based on its ability to pass through the blood-brain barrier, 2X-121 has potential to treat brain metastases and primary brain tumors.
A Phase 2, open-label clinical trial is ongoing, to investigate the anti-tumor effect and tolerability of 2X-121 in 30 heavily pre-treated metastatic breast cancer patients regardless of hormone receptor, HER2, or BRCA1 or 2 status, who have relapsed on two or more different prior therapies and who are identified by the 2X-121 DRP as highly likely to respond to treatment with 2X-121. The first patient was dosed in June 2018.
The first U.S. patient has now been dosed at Dana-Farber Cancer Institute Boston with 2X-121, a PARP inhibitor in development for advanced ovarian cancer. 2X-121 has previously shown promising results in ovarian cancer patients in a phase 1 study performed by EISAI. The DRP® selection, aimed to find the best responding patients, is under development in order to achieve an increased response rate in order to outperform currently marketed drugs for the same indication.
Additionally, OV-U.S. is collaborating with the NCI Pediatric Preclinical Testing Consortium (PPTC), funded by the National Cancer Institute (NCI), part of the National Institutes of Health (NIH), to evaluate 2X-121 in pediatric cancers, including rare cancer types seen by the NCI, assuming 2X-121 demonstrates activity in the PPTC models. Positive response prediction signals have been identified, and preclinical studies are underway to evaluate 2X-121 against in vivo panels of certain pediatric tumors including neuroblastoma, sarcoma and renal tumors using different mouse models like SCID or other immunodeficient mice strains.
2X-121 was previously evaluated in an open label, multi-center Phase 1/2 dose-ranging study in 41 patients with advanced solid tumors. Two patients had partial response and 13 patients had stable disease, with eight of these >24 weeks. No significant hematological toxicity and no Grade 4 or 5 adverse events were seen. The study abstract was presented at the 2018 the American Society for Clinical Oncology Clinical Meeting.
The ongoing and planned studies using the 2X-121 DRP are expected to significantly improve response rates seen in this first-in-human study.
A molecular model of 2X-121
The mRNA-driven multiple biomarker DRP for this product candidate was validated in 2017 in a 13-patient blinded study. The DRP correctly predicted response to treatment with 2X-121 and overall survival with a p-value of 0.07 and a hazard ratio on overall survival of 0.26 in this study. Notably, this study also established the ability of the 2X-121 DRP to predict responders irrespective of BRCA mutation status (Plummer ER et al 2018; Access abstract).
Plummer ER et al. First-in-human phase 1 study of the PARP/tankyrase inhibitor 2X-121 (E7449) as monotherapy in patients with advanced solid tumors and validation of a novel drug response predictor (DRP) mRNA biomarker. J Clin Oncol 36, 2018 (suppl; abstr 2505).